Rapid in-solution equilibrium approach to K_D determination of biotherapeutic drug candidates 

Johan Engström, senior scientist at Gyros Protein Technologies, presents aspects of in-solution equilibrium affinity experiments using Gyrolab® systems.

Determination of binding affinity and kinetics of antibody-target interactions is essential in bringing therapeutic antibodies from discovery to the market. Gyrolab systems can be used to quickly generate in-solution titration curves in a relevant matrix that mimics biological systems and with interactants in their native form.

An introduction to the set up and evaluation of classical in-solution equilibrium experiments for determination of sub-nanomolar K_D values and active concentrations of interactants is discussed. Examples of determinations of picomolar affinities for several marketed TNFα antagonists.

Benefits of listening to the webinar:

• Learn how to determine sub-nanomolar K_D values within an hour
• Find out how to set up in-solution equilibrium experiments using intuitive software
• Learn how results are fitted using a novel kinetic model to obtain K_D values and active interactant concentrations