Rapid in-solution equilibrium approach to KD determination of biotherapeutic drug candidates
Johan Engström, senior scientist at Gyros Protein Technologies, presents aspects of in-solution equilibrium affinity experiments using Gyrolab™ systems. Examples of determinations of picomolar affinities for several marketed TNFα antagonists will be discussed.
Introduction to setting up and evaluating classical in-solution equilibrium experiments for determination of sub-nanomolar KD’s and active concentrations of interactants
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